Cal is a heterodimer, which is composed of MRP 8 and MRP 14. It exists in neutrophils cytoplasm and expressed on mononuclear cell membranes. Cal is acute phase proteins, it has a well stable phase about one week in human faeces, it is determined to be a inflammatory bowel disease marker[2-3]. The kit is a simple, visual quantitative test that detects Cal in human faeces, it has high detection sensitivity and strong specificity. The test based on high specificit of double antibodies sandwich reaction principle and fluorescence immunochromatographic assay analysis technics, which can give a result within 15 minutes.
Principle of The procedure
The strip has anti-Cal coating antibody on test region, which is fastened to membrane chromatography in advance. Lable pad is coated by fluorescence labeled anti-Cal antibody in advance. When testing positive sample, the Cal in sample can be mixed with fluorescence labeled anti-Cal antibody, and form immune mixture. As the mixture is allowed to migrate along the test strip, the Cal conjugate complex is captured by anti-Cal coating antibody on the membrane and forms complex. The fluorescence intensity is positively correlated with the Cal content. The Cal in sample can be detected by fluorescence immunoassay analyzer.